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2013;27(8):398405 Hartweg J, Farmer AJ, Holman RR, and Neil HAW

Once reaching near-confluence, culture medium was aspirated and cells were washed once with PBS, followed by a 20 min incubation with NucBlue Live ReadyProbes Reagent (#R37605, ThermoFisher Scientific, Waltham, MA, USA) to stain the nuclei